Hotspot

atpH-atpI

LSC · 14861-15986 · 1126 bp

Back to Exbucklandia

Pi 0.00178

MarkerSeek score 60.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1126
variable_sites: 3
pi: 0.00178
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	45.0	55.0	58.0	57.8	1183 bp	1.0	78.7
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	CGAATCCATGGAGGGTCATC	50.0	55.0	57.9	57.8	1239 bp	1.0	78.1
atpH-atpI_p3	AATAACGGAAGCGGCAGAAA	CGAATCCATGGAGGGTCATC	45.0	55.0	58.2	57.8	1184 bp	1.0	77.5
atpH-atpI_p4	GCAGTACCTTGACCAACTCC	CGAATCCATGGAGGGTCATC	55.0	55.0	58.2	57.8	1243 bp	1.0	77.5
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	CGAATCCATGGAGGGTCATC	50.0	55.0	57.8	57.8	1198 bp	1.0	77.3