Hotspot

matK-trnK-UUU

LSC · 3630-4333 · 704 bp

Back to Exbucklandia

Pi 0.00284

MarkerSeek score 60.60

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 704
variable_sites: 3
pi: 0.00284
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	CCATGATCATGAGCAAGTGC	AACGGTAGAGTACTCGGCTT	50.0	50.0	57.2	58.2	835 bp	1.0	72.7
matK-trnK-UUU_p2	CCATGATCATGAGCAAGTGC	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.2	58.2	834 bp	1.0	72.7
matK-trnK-UUU_p3	CCATGATCATGAGCAAGTGC	GCGACTGTATCTACACACCC	50.0	55.0	57.2	57.8	891 bp	1.0	72.4
matK-trnK-UUU_p4	CCATGATCATGAGCAAGTGC	CGTCGTGCAATCCAATCTCT	50.0	50.0	57.2	58.4	926 bp	1.0	71.2
matK-trnK-UUU_p5	CCATGATCATGAGCAAGTGC	GGGTTGCTAACTCAACGGTA	50.0	50.0	57.2	57.5	848 bp	1.0	70.4