Hotspot

trnK-UUU

LSC · 154161-154196 · 36 bp

Back to Evolvulus

Pi 0.639
MarkerSeek score 72.60
Primer available yes
Label trnK-UUU

Feature Metadata

label_name
trnK-UUU
feature_type
tRNA
region
LSC
length_bp
36
variable_sites
23
pi
0.639
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU_p1 ATCGTCAAATCTCGCTTGCT TGGCTTTAAAAGGGACGCTT 45.0 45.0 58.0 58.0 1038 bp 0.5 59.8
trnK-UUU_p2 ATCGTCAAATCTCGCTTGCT TTGGCTTTAAAAGGGACGCT 45.0 45.0 58.0 58.0 1039 bp 0.5 59.8
trnK-UUU_p3 GTTGAACCCTGGAAATTGCG TGGCTTTAAAAGGGACGCTT 50.0 45.0 57.9 58.0 1017 bp 0.5 59.4
trnK-UUU_p4 GTTGAACCCTGGAAATTGCG TTGGCTTTAAAAGGGACGCT 50.0 45.0 57.9 58.0 1018 bp 0.5 59.4
trnK-UUU_p5 ATCGTCAAATCTCGCTTGCT CCAGCCATTCCCTTGACTTT 45.0 50.0 58.0 58.1 921 bp 0.5 59.3
trnK-UUU_p1 TGAATTCAATTGGGTCCACGA GGTGGAACTTCGGCCTAATC 42.9 55.0 57.9 58.3 2969 bp 0.5 48.1
trnK-UUU_p2 TCAATTGGGTCCACGAATCT GGTGGAACTTCGGCCTAATC 45.0 55.0 56.8 58.3 2964 bp 0.5 47.9
trnK-UUU_p3 TGAATTCAATTGGGTCCACGA GTGGAACTTCGGCCTAATCA 42.9 50.0 57.9 57.6 2968 bp 0.5 47.6
trnK-UUU_p4 TCAATTGGGTCCACGAATCT GTGGAACTTCGGCCTAATCA 45.0 50.0 56.8 57.6 2963 bp 0.5 47.5
trnK-UUU_p5 TGTGAATGAAGGATCCCACTG GGTGGAACTTCGGCCTAATC 47.6 55.0 57.6 58.3 2988 bp 0.5 46.5
trnK-UUU_p1 GAAGAACCAAAGCTCGTTGC GGTGGAACTTCGGCCTAATC 50.0 55.0 58.0 58.3 722-1075 bp 1.0 88.0
trnK-UUU_p2 AGCCGAATCGCTCTTTTGAT GGTGGAACTTCGGCCTAATC 45.0 55.0 58.0 58.3 921-1275 bp 1.0 87.6
trnK-UUU_p3 TCTCCTTCGAATCCCCCTTT GGTGGAACTTCGGCCTAATC 50.0 55.0 58.0 58.3 1023 bp 0.5 57.1
trnK-UUU_p4 AGTCGCGGTCTTACAAACTC GGTGGAACTTCGGCCTAATC 50.0 55.0 57.9 58.3 517 bp 0.5 56.8
trnK-UUU_p5 GTCGCGGTCTTACAAACTCT GGTGGAACTTCGGCCTAATC 50.0 55.0 57.9 58.3 516 bp 0.5 56.8