Hotspot

matK

LSC · 154468-155778 · 1311 bp

Back to Evolvulus

Pi 0.459

MarkerSeek score 82.70

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1311
variable_sites: 578
pi: 0.459
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	ATCGTCAAATCTCGCTTGCT	AAAGGGGGATTCGAAGGAGA	45.0	50.0	58.0	58.0	2091 bp	0.5	59.6
matK_p2	ATCGTCAAATCTCGCTTGCT	GCAACGAGCTTTGGTTCTTC	45.0	50.0	58.0	58.0	2392 bp	0.5	59.6
matK_p3	ATCGTCAAATCTCGCTTGCT	AGAGTTTGTAAGACCGCGAC	45.0	50.0	58.0	57.9	2598 bp	0.5	59.4
matK_p4	ATCGTCAAATCTCGCTTGCT	GAGTTTGTAAGACCGCGACT	45.0	50.0	58.0	57.9	2597 bp	0.5	59.4
matK_p5	ATCGTCAAATCTCGCTTGCT	TCTGCCTTTGGTCCAAATCC	45.0	50.0	58.0	58.1	1941 bp	0.5	59.3