Hotspot

clpP

LSC · 66323-68523 · 2201 bp

Back to Evolvulus

Pi 0.00505

MarkerSeek score 63.60

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2201
variable_sites: 10
pi: 0.00505
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	GAATTCGAAGTGCCATGCTA	CAAAACCCTACGTTTCCCCA	45.0	50.0	56.2	58.0	2244-2412 bp	1.0	70.0
clpP_p2	GAATTCGAAGTGCCATGCTA	TCAAAACCCTACGTTTCCCC	45.0	50.0	56.2	57.7	2245-2413 bp	1.0	67.9
clpP_p3	GAATTCGAAGTGCCATGCTAT	CAAAACCCTACGTTTCCCCA	42.9	50.0	56.7	58.0	2244-2412 bp	1.0	66.2
clpP_p4	AATTCGAAGTGCCATGCTAT	CAAAACCCTACGTTTCCCCA	40.0	50.0	55.5	58.0	2243-2411 bp	1.0	64.2
clpP_p5	ATTCGAAGTGCCATGCTATT	CAAAACCCTACGTTTCCCCA	40.0	50.0	55.5	58.0	2242-2410 bp	1.0	64.2