Hotspot

trnK-UUU-rps16

LSC · 156586-156947 · 362 bp

Back to Evolvulus

Pi 0.4

MarkerSeek score 75.70

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 362
variable_sites: 142
pi: 0.4
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	GAAGAACCAAAGCTCGTTGC	GGTGGAACTTCGGCCTAATC	50.0	55.0	58.0	58.3	722-1075 bp	1.0	88.0
trnK-UUU-rps16_p2	AGCCGAATCGCTCTTTTGAT	GGTGGAACTTCGGCCTAATC	45.0	55.0	58.0	58.3	921-1275 bp	1.0	87.6
trnK-UUU-rps16_p3	TCTCCTTCGAATCCCCCTTT	GGTGGAACTTCGGCCTAATC	50.0	55.0	58.0	58.3	1023 bp	0.5	57.1
trnK-UUU-rps16_p4	AGTCGCGGTCTTACAAACTC	GGTGGAACTTCGGCCTAATC	50.0	55.0	57.9	58.3	517 bp	0.5	56.8
trnK-UUU-rps16_p5	GTCGCGGTCTTACAAACTCT	GGTGGAACTTCGGCCTAATC	50.0	55.0	57.9	58.3	516 bp	0.5	56.8