Hotspot

trnL-CAA-ycf2

LSC · 147564-147724 · 161 bp

Back to Evolvulus

Pi 0.0435

MarkerSeek score 70.10

Primer available yes

Label trnL-CAA-ycf2

Feature Metadata

label_name: trnL-CAA-ycf2
feature_type: igs
region: LSC
length_bp: 161
variable_sites: 7
pi: 0.0435
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ycf2_p1	AAATGGTAGACACGCGAGAC	ATGGCTTTTCCCGGATGAAA	50.0	45.0	58.0	57.8	270 bp	1.0	80.2
trnL-CAA-ycf2_p2	AAATGGTAGACACGCGAGAC	TGGCTTTTCCCGGATGAAAT	50.0	45.0	58.0	57.8	269 bp	1.0	80.2
trnL-CAA-ycf2_p3	TGAAATGGTAGACACGCGAG	TGGCTTTTCCCGGATGAAAT	50.0	45.0	58.0	57.8	271 bp	1.0	80.2
trnL-CAA-ycf2_p4	TGAAATGGTAGACACGCGAG	ATGGCTTTTCCCGGATGAAA	50.0	45.0	58.0	57.8	272 bp	1.0	80.1
trnL-CAA-ycf2_p5	AAATGGTAGACACGCGAGAC	AAGATGGCTTTTCCCGGATG	50.0	50.0	58.0	58.2	273 bp	1.0	80.1