Hotspot

trnI-CAU-ycf2

LSC · 82671-83203 · 533 bp

Back to Evolvulus

Pi 0.00772

MarkerSeek score 64.50

Primer available yes

Label trnI-CAU-ycf2

Feature Metadata

label_name: trnI-CAU-ycf2
feature_type: igs
region: LSC
length_bp: 533
variable_sites: 4
pi: 0.00772
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-ycf2_p1	ATTGAACCTACGACTTCGCC	TGAATTCAATTGGGTCCACGA	50.0	42.9	58.0	57.9	635-650 bp	1.0	73.1
trnI-CAU-ycf2_p2	GAACCTACGACTTCGCCAAT	TGAATTCAATTGGGTCCACGA	50.0	42.9	58.0	57.9	632-647 bp	1.0	73.1
trnI-CAU-ycf2_p3	ATTGAACCTACGACTTCGCC	TCAATTGGGTCCACGAATCT	50.0	45.0	58.0	56.8	630-645 bp	1.0	73.0
trnI-CAU-ycf2_p4	GAACCTACGACTTCGCCAAT	TCAATTGGGTCCACGAATCT	50.0	45.0	58.0	56.8	627-642 bp	1.0	73.0
trnI-CAU-ycf2_p5	GATGGTGTGCATCCAGTAGG	TGAATTCAATTGGGTCCACGA	55.0	42.9	58.0	57.9	656-671 bp	1.0	72.8