Hotspot

rrn16

IRa · 100576-102072 · 1497 bp

Back to Eutreptiella

Pi 0.162

MarkerSeek score 81.20

Primer available yes

Label rrn16

Feature Metadata

label_name: rrn16
feature_type: rRNA
region: IRa
length_bp: 1497
variable_sites: 237
pi: 0.162
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16_p1	GACTTGACGTCATCCTCACC	GCCGACACTGACACTAAGAG	55.0	55.0	58.0	58.0	452 bp	0.5	59.9
rrn16_p2	GACTTGACGTCATCCTCACC	GAAAGCCTGACGGAGCAATA	55.0	50.0	58.0	58.0	792 bp	0.5	59.8
rrn16_p3	GACTTGACGTCATCCTCACC	GGCAGTGGAAACTACTAGGC	55.0	55.0	58.0	58.0	563 bp	0.5	59.8
rrn16_p4	AGATCGCAACTCATTGTCCC	GCCGACACTGACACTAAGAG	50.0	55.0	58.0	58.0	516 bp	0.5	59.6
rrn16_p5	GACTTGACGTCATCCTCACC	GGTAATACGGAGGGTGCAAG	55.0	55.0	58.0	58.1	666 bp	0.5	59.6