Hotspot

OF286_pgp058

LSC · 23964-25391 · 1428 bp

Back to Eutreptiella

Pi 0.147
MarkerSeek score 82.90
Primer available yes
Label OF286_pgp058

Feature Metadata

label_name
OF286_pgp058
feature_type
gene
region
LSC
length_bp
1428
variable_sites
193
pi
0.147
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
OF286_pgp058_p1 ATGGGTGGTTTTGCGTTAGT CAGTACCACCAGTAGCGTTC 45.0 55.0 58.0 58.0 2853 bp 0.5 59.9
OF286_pgp058_p2 ATGGGTGGTTTTGCGTTAGT GTACCACCAGTAGCGTTCAG 45.0 55.0 58.0 58.0 2851 bp 0.5 59.9
OF286_pgp058_p3 ATGGGTGGTTTTGCGTTAGT CGTATACTGCACGACCGTAG 45.0 55.0 58.0 58.0 2685 bp 0.5 59.8
OF286_pgp058_p4 TGATGGGTGGTTTTGCGTTA CAGTACCACCAGTAGCGTTC 45.0 55.0 58.0 58.0 2855 bp 0.5 59.8
OF286_pgp058_p5 TGATGGGTGGTTTTGCGTTA GTACCACCAGTAGCGTTCAG 45.0 55.0 58.0 58.0 2853 bp 0.5 59.8