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OF286_pgp055

LSC · 28446-29279 · 834 bp

Back to Eutreptiella

Pi 0.269

MarkerSeek score 79.90

Primer available yes

Label OF286_pgp055

Feature Metadata

label_name: OF286_pgp055
feature_type: gene
region: LSC
length_bp: 834
variable_sites: 222
pi: 0.269
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
OF286_pgp055_p1	GTCGCGTGACACCTTAGTAG	CGCTCTTAACATGACCACGA	55.0	50.0	58.1	58.0	1536 bp	0.5	59.1
OF286_pgp055_p2	GTCGCGTGACACCTTAGTAG	TCGCTCTTAACATGACCACG	55.0	50.0	58.1	58.0	1537 bp	0.5	59.1
OF286_pgp055_p3	TATATTCGTCGCGTGACACC	CGCTCTTAACATGACCACGA	50.0	50.0	57.9	58.0	1543 bp	0.5	59.0
OF286_pgp055_p4	TATATTCGTCGCGTGACACC	TCGCTCTTAACATGACCACG	50.0	50.0	57.9	58.0	1544 bp	0.5	59.0
OF286_pgp055_p5	GTCGCGTGACACCTTAGTAG	GACACTACGCTTCTCACCAG	55.0	55.0	58.1	58.0	1367 bp	0.5	59.0