Hotspot

OF286_pgp048

LSC · 34568-35107 · 540 bp

Back to Eutreptiella

Pi 0.276

MarkerSeek score 78.80

Primer available yes

Label OF286_pgp048

Feature Metadata

label_name: OF286_pgp048
feature_type: gene
region: LSC
length_bp: 540
variable_sites: 147
pi: 0.276
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
OF286_pgp048_p1	GGGTGTTGCGGAGTTAGTAG	ACAGACGCACGAACTTTCAT	55.0	45.0	58.0	58.1	1860 bp	0.5	58.9
OF286_pgp048_p2	CGGATGTTGTTAAGGCCGTA	ACAGACGCACGAACTTTCAT	50.0	45.0	58.0	58.1	1485 bp	0.5	58.8
OF286_pgp048_p3	CTGAAGGTGCTCCAAAAGGT	ACAGACGCACGAACTTTCAT	50.0	45.0	58.0	58.1	1383 bp	0.5	58.8
OF286_pgp048_p4	CGAATGGGTTCTGGTAAGGG	ACAGACGCACGAACTTTCAT	55.0	45.0	58.0	58.1	1937 bp	0.5	58.6
OF286_pgp048_p5	TACTCCCAACATGCCTCTGA	ACAGACGCACGAACTTTCAT	50.0	45.0	58.0	58.1	1511 bp	0.5	58.5