Hotspot

OF286_pgp042

LSC · 39755-40810 · 1056 bp

Back to Eutreptiella

Pi 0.482

MarkerSeek score 82.80

Primer available yes

Label OF286_pgp042

Feature Metadata

label_name: OF286_pgp042
feature_type: gene
region: LSC
length_bp: 1056
variable_sites: 467
pi: 0.482
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
OF286_pgp042_p1	AACGCTCACAACTTCCCATT	GAATCTGTACAGCACGAGCA	45.0	50.0	58.0	58.0	2351-2804 bp	1.0	91.8
OF286_pgp042_p2	AACGCTCACAACTTCCCATT	AGTCCAGACCAACCTACGAA	45.0	50.0	58.0	58.0	2040 bp	0.5	59.8
OF286_pgp042_p3	AGCTAGTTGTCGTAAGTGCG	GAATCTGTACAGCACGAGCA	50.0	50.0	58.0	58.0	2077 bp	0.5	59.8
OF286_pgp042_p4	AGCTAGTTGTCGTAAGTGCG	AGTCCAGACCAACCTACGAA	50.0	50.0	58.0	58.0	1766 bp	0.5	59.8
OF286_pgp042_p5	GCTAGTTGTCGTAAGTGCGA	GAATCTGTACAGCACGAGCA	50.0	50.0	58.0	58.0	2076 bp	0.5	59.8