Hotspot

OF286_pgp002

LSC · 92213-95179 · 2967 bp

Back to Eutreptiella

Pi 0.467

MarkerSeek score 71.50

Primer available yes

Label OF286_pgp002

Feature Metadata

label_name: OF286_pgp002
feature_type: gene
region: LSC
length_bp: 2967
variable_sites: 871
pi: 0.467
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.63
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
OF286_pgp002_p1	TTATGGTACTGCACGAACGG	TCATGGGGTGCGTATATCCT	50.0	50.0	58.0	58.0	993 bp	0.5	59.8
OF286_pgp002_p2	TTATGGTACTGCACGAACGG	CGCATAGTTAGTTGGGTCCC	50.0	55.0	58.0	58.1	930 bp	0.5	59.6
OF286_pgp002_p3	TTATGGTACTGCACGAACGG	GCATAGTTAGTTGGGTCCCG	50.0	55.0	58.0	58.1	929 bp	0.5	59.6
OF286_pgp002_p4	AATGCGAGGTCTTATGGCAG	TCATGGGGTGCGTATATCCT	50.0	50.0	58.0	58.0	636 bp	0.5	59.5
OF286_pgp002_p5	GAATGCGAGGTCTTATGGCA	TCATGGGGTGCGTATATCCT	50.0	50.0	58.0	58.0	637 bp	0.5	59.4