Hotspot

OF286_pgp060-OF286_pgp059

LSC · 22160-23261 · 1102 bp

Back to Eutreptiella

Pi 0.176

MarkerSeek score 80.90

Primer available yes

Label OF286_pgp060-OF286_pgp059

Feature Metadata

label_name: OF286_pgp060-OF286_pgp059
feature_type: igs
region: LSC
length_bp: 1102
variable_sites: 164
pi: 0.176
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
OF286_pgp060-OF286_pgp059_p1	AGTCAAATTCGTGCTTGGGT	ACTAACGCAAAACCACCCAT	45.0	45.0	58.0	58.0	1857 bp	0.5	59.8
OF286_pgp060-OF286_pgp059_p2	AGTCAAATTCGTGCTTGGGT	TAACGCAAAACCACCCATCA	45.0	45.0	58.0	58.0	1855 bp	0.5	59.7
OF286_pgp060-OF286_pgp059_p3	AGTCAAATTCGTGCTTGGGT	GCCCTGTCAGAGTTGGAAAA	45.0	50.0	58.0	58.0	1739 bp	0.5	59.7
OF286_pgp060-OF286_pgp059_p4	AGTCAAATTCGTGCTTGGGT	TCCTGCGCCTGTTTTAGTTT	45.0	45.0	58.0	58.0	2356 bp	0.5	59.7
OF286_pgp060-OF286_pgp059_p5	AGTCAAATTCGTGCTTGGGT	TTCCGCAGCTTTCTCAAACT	45.0	45.0	58.0	58.0	1975 bp	0.5	59.6