Hotspot

ccsA-ndhD

SSC · 114717-114942 · 226 bp

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Pi 0.0491

MarkerSeek score 72.00

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 226
variable_sites: 26
pi: 0.0491
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CTCTCCAGTCCCAGTGGTTA	TTATCGATGGCTTCCCTTGC	55.0	50.0	58.1	58.0	1163-1179 bp	1.0	84.8
ccsA-ndhD_p2	CTCTCCAGTCCCAGTGGTTA	CCTTGGTTGATGGTAGTCGG	55.0	55.0	58.1	58.0	1472-1488 bp	1.0	84.2
ccsA-ndhD_p3	CTCTCCAGTCCCAGTGGTTA	GATCCTGGGCTTAATGGAGC	55.0	55.0	58.1	58.1	1331-1347 bp	1.0	83.9
ccsA-ndhD_p4	AAATGCCTCAGCCTGCAATA	CCTTGGTTGATGGTAGTCGG	45.0	55.0	57.9	58.0	1502-1518 bp	1.0	83.4
ccsA-ndhD_p5	CTCTCCAGTCCCAGTGGTTA	CCTGGGCTTAATGGAGCAAT	55.0	50.0	58.1	57.9	1344 bp	0.375	54.1