Hotspot

trnP-TGG-psaJ

LSC · 66076-66413 · 338 bp

Back to Eutrema

Pi 0.0302
MarkerSeek score 70.20
Primer available yes
Label trnP-TGG-psaJ

Feature Metadata

label_name
trnP-TGG-psaJ
feature_type
igs
region
LSC
length_bp
338
variable_sites
27
pi
0.0302
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-TGG-psaJ_p1 ACCCGTGACATTTTGTACCC AAACCTGCTAACGAACCGAA 50.0 45.0 58.1 57.8 467-476 bp 1.0 82.7
trnP-TGG-psaJ_p2 CCCGTGACATTTTGTACCCA AAACCTGCTAACGAACCGAA 50.0 45.0 58.1 57.8 466-475 bp 1.0 82.7
trnP-TGG-psaJ_p3 ACAGGATTTGAACCCGTGAC AAACCTGCTAACGAACCGAA 50.0 45.0 58.1 57.8 478-487 bp 1.0 82.5
trnP-TGG-psaJ_p4 CAGGATTTGAACCCGTGACA AAACCTGCTAACGAACCGAA 50.0 45.0 58.1 57.8 477-486 bp 1.0 82.5
trnP-TGG-psaJ_p5 TGACAGGATTTGAACCCGTG AAACCTGCTAACGAACCGAA 50.0 45.0 58.1 57.8 480-489 bp 1.0 82.5