Hotspot

psbE-petL

LSC · 63990-65347 · 1358 bp

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Pi 0.0285

MarkerSeek score 66.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1358
variable_sites: 89
pi: 0.0285
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	CCGAAATAACTAGTTATAGTAGGCA	50.0	36.0	57.8	56.0	1005-1453 bp	1.0	49.2
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	CCGAAATAACTAGTTATAGTAGGCA	55.0	36.0	58.2	56.0	1000-1448 bp	1.0	49.2
psbE-petL_p3	TCTCCTGTGCTTCCAGACAT	CCGAAATAACTAGTTATAGTAGGCA	50.0	36.0	58.4	56.0	998-1446 bp	1.0	49.2
psbE-petL_p4	TTCTCCTGTGCTTCCAGACA	CCGAAATAACTAGTTATAGTAGGCA	50.0	36.0	58.6	56.0	999-1447 bp	1.0	49.2
psbE-petL_p5	AAGAACGTTCTCCTGTGCTT	CCGAAATAACTAGTTATAGTAGGCA	45.0	36.0	57.4	56.0	1006-1454 bp	1.0	49.2