Hotspot

ndhC-trnM-CAT

LSC · 49969-51724 · 1756 bp

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Pi 0.0228

MarkerSeek score 71.80

Primer available yes

Label ndhC-trnM-CAT

Feature Metadata

label_name: ndhC-trnM-CAT
feature_type: igs
region: LSC
length_bp: 1756
variable_sites: 104
pi: 0.0228
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnM-CAT_p1	TGCCAAAACAGGAATAGCACT	TTGAACCAATGACTCCTGCC	42.9	50.0	58.1	58.1	1738-1912 bp	1.0	78.7
ndhC-trnM-CAT_p2	TGCCAAAACAGGAATAGCACT	CTCCTGCCGTATGAAAGCAA	42.9	50.0	58.1	58.3	1726-1900 bp	1.0	77.4
ndhC-trnM-CAT_p3	TGCCAAAACAGGAATAGCAC	TTGAACCAATGACTCCTGCC	45.0	50.0	56.6	58.1	1738-1912 bp	1.0	76.6
ndhC-trnM-CAT_p4	TGCCAAAACAGGAATAGCACT	CCAATGACTCCTGCCGTATG	42.9	55.0	58.1	58.4	1733-1907 bp	1.0	76.2
ndhC-trnM-CAT_p5	TGCCAAAACAGGAATAGCACT	TCCTGCCGTATGAAAGCAAT	42.9	45.0	58.1	57.6	1725-1899 bp	1.0	76.1