Hotspot

trnT-TGT-trnL-CAA

LSC · 46115-47657 · 1543 bp

Back to Eutrema

Pi 0.0212

MarkerSeek score 64.30

Primer available yes

Label trnT-TGT-trnL-CAA

Feature Metadata

label_name: trnT-TGT-trnL-CAA
feature_type: igs
region: LSC
length_bp: 1543
variable_sites: 81
pi: 0.0212
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-TGT-trnL-CAA_p1	CGGAGCGTGATTGGTTTCTA	AAAGCCGAGCCAATAGGATG	50.0	50.0	58.0	58.0	2462-2979 bp	0.875	82.7
trnT-TGT-trnL-CAA_p2	TTTTCCGGCGATACGAACAT	CATCCACGACTGAAACGGAT	45.0	50.0	58.0	58.0	2823-2954 bp	0.875	81.2
trnT-TGT-trnL-CAA_p3	TTTTCCGGCGATACGAACAT	AAAGCCGAGCCAATAGGATG	45.0	50.0	58.0	58.0	2569-2690 bp	0.875	81.2
trnT-TGT-trnL-CAA_p4	CGGAGCGTGATTGGTTTCTA	CATCCACGACTGAAACGGAT	50.0	50.0	58.0	58.0	2716-2847 bp	0.75	76.2
trnT-TGT-trnL-CAA_p5	CGGAGCGTGATTGGTTTCTA	CCGAAAGTTGGATAGGGTGG	50.0	55.0	58.0	58.0	2688-2819 bp	0.75	76.0