Hotspot

petN-psbM

LSC · 28159-28705 · 547 bp

Back to Eutrema

Pi 0.0288

MarkerSeek score 71.30

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 547
variable_sites: 41
pi: 0.0288
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCGTAGTGTGGGGAAGAAGT	TGCTACTGCACTCTTCATTCT	50.0	42.9	58.0	57.0	650-667 bp	1.0	70.3
petN-psbM_p2	CGTAGTGTGGGGAAGAAGTG	TGCTACTGCACTCTTCATTCT	55.0	42.9	57.9	57.0	649-666 bp	1.0	69.7
petN-psbM_p3	CTCTCGTAGTGTGGGGAAGA	TGCTACTGCACTCTTCATTCT	55.0	42.9	58.2	57.0	653-670 bp	1.0	69.0
petN-psbM_p4	CTCTCTCGTAGTGTGGGGAA	TGCTACTGCACTCTTCATTCT	55.0	42.9	58.2	57.0	655-672 bp	1.0	69.0
petN-psbM_p5	TCTCTCGTAGTGTGGGGAAG	TGCTACTGCACTCTTCATTCT	55.0	42.9	58.2	57.0	654-671 bp	1.0	69.0