Hotspot

psbK-psbI

LSC · 7245-7680 · 436 bp

Back to Eutrema

Pi 0.0453

MarkerSeek score 85.90

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 436
variable_sites: 45
pi: 0.0453
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 85.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCAGCTGTAAG	TTCTTCACGTCCCGGATTAC	50.0	50.0	57.9	57.3	569-587 bp	1.0	83.0
psbK-psbI_p2	TGGCAAGCAGCTGTAAGTTT	TTCTTCACGTCCCGGATTAC	45.0	50.0	58.3	57.3	566-584 bp	1.0	81.1
psbK-psbI_p3	TTGGCAAGCAGCTGTAAGTT	TTCTTCACGTCCCGGATTAC	45.0	50.0	58.3	57.3	567-585 bp	1.0	81.0
psbK-psbI_p4	TTTGGCAAGCAGCTGTAAGT	TTCTTCACGTCCCGGATTAC	45.0	50.0	58.3	57.3	568-586 bp	1.0	81.0
psbK-psbI_p5	GTTTGGCAAGCAGCTGTAAG	CCGGATTACGTCCTGGATCA	50.0	55.0	57.9	59.0	558-576 bp	1.0	80.8