Hotspot

matK-rps16

LSC · 3648-5100 · 1453 bp

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Pi 0.023

MarkerSeek score 65.50

Primer available yes

Label matK-rps16

Feature Metadata

label_name: matK-rps16
feature_type: igs
region: LSC
length_bp: 1453
variable_sites: 79
pi: 0.023
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	CGAGCCCCATCGAACTTTAA	CCCAATGAGCCGTTTATCGA	50.0	50.0	58.0	58.1	1525-1774 bp	0.875	82.5
matK-rps16_p2	CGAGCCCCATCGAACTTTAA	TCCCAATGAGCCGTTTATCG	50.0	50.0	58.0	58.1	1526-1775 bp	0.875	82.5
matK-rps16_p3	CGAGCCCCATCGAACTTTAA	ATGCGTGAATCAAGCGTTTG	50.0	45.0	58.0	58.0	2070-2316 bp	0.875	82.4
matK-rps16_p4	CGAGCCCCATCGAACTTTAA	CATGCGTGAATCAAGCGTTT	50.0	45.0	58.0	58.0	2071-2317 bp	0.875	82.4
matK-rps16_p5	CGAGCCCCATCGAACTTTAA	TCGATCCCGAAGAGAAGGAA	50.0	50.0	58.0	57.9	1488-1737 bp	0.875	81.9