Hotspot

atpF

LSC · 12258-13600 · 1343 bp

Back to Euterpe

Pi 0.00347

MarkerSeek score 61.00

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1343
variable_sites: 7
pi: 0.00347
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	ACTAATTTCGTCGGCTCGAA	AGAACTCTGTTCTTTGTTAGTCCT	45.0	37.5	57.4	57.5	1495-1502 bp	1.0	40.4
atpF_p2	ACTAATTTCGTCGGCTCGAA	AGTCCTATCTATAAGAGGAGAGCA	45.0	41.7	57.4	57.3	1477-1484 bp	1.0	40.4
atpF_p3	GGCTCGAAGGGTTACCATTA	AGAACTCTGTTCTTTGTTAGTCCT	50.0	37.5	57.0	57.5	1483-1490 bp	1.0	40.4
atpF_p4	ACTAATTTCGTCGGCTCGAA	AGAACTCTGTTCTTTGTTAGTCC	45.0	39.1	57.4	56.2	1495-1502 bp	1.0	40.4
atpF_p5	ACTAATTTCGTCGGCTCGAA	GAACTCTGTTCTTTGTTAGTCCT	45.0	39.1	57.4	56.2	1494-1501 bp	1.0	40.4