Hotspot

ndhF-rpl32

SSC · 116038-116369 · 332 bp

Back to Euterpe

Pi 0.012
MarkerSeek score 54.20
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
332
variable_sites
6
pi
0.012
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TTGTTGCTGTCGGAATAAGT CGATTTCGCTAAAGAAAAAGCT 40.0 36.4 55.3 56.0 543-584 bp 1.0 40.9
ndhF-rpl32_p2 TTGTTGCTGTCGGAATAAGT CGCTAAAGAAAAAGCTTTTACTGC 40.0 37.5 55.3 57.9 537-578 bp 1.0 40.9
ndhF-rpl32_p3 TTTGTTGCTGTCGGAATAAGT CGATTTCGCTAAAGAAAAAGCT 38.1 36.4 56.1 56.0 544-585 bp 1.0 40.9
ndhF-rpl32_p4 TTGTTGCTGTCGGAATAAGT CGATTTCGCTAAAGAAAAAGCTT 40.0 34.8 55.3 56.7 543-584 bp 1.0 40.9
ndhF-rpl32_p5 TTTGTTGCTGTCGGAATAAGT CGCTAAAGAAAAAGCTTTTACTGC 38.1 37.5 56.1 57.9 538-579 bp 1.0 40.9