Hotspot

psbM-trnD-GUC

LSC · 30851-31849 · 999 bp

Back to Euterpe

Pi 0.00267

MarkerSeek score 60.50

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 999
variable_sites: 4
pi: 0.00267
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	AGTTCAATCGGTCAGAGCAC	45.0	50.0	55.8	57.9	1112-1113 bp	1.0	61.8
psbM-trnD-GUC_p2	AGAATGAACAGTGCAGTAGC	TATGCCATACACCTTGCTGG	45.0	50.0	55.8	57.7	1138-1139 bp	1.0	59.7
psbM-trnD-GUC_p3	AGAATGAACAGTGCAGTAGC	CCATACACCTTGCTGGGATT	45.0	50.0	55.8	57.6	1134-1135 bp	1.0	58.9
psbM-trnD-GUC_p4	AGGAACTAGAATGAACAGTGCA	AGTTCAATCGGTCAGAGCAC	40.9	50.0	57.4	57.9	1119-1120 bp	1.0	58.6
psbM-trnD-GUC_p5	AGAATGAACAGTGCAGTAGC	CACCTTGCTGGGATTGTAGT	45.0	50.0	55.8	57.5	1129-1130 bp	1.0	58.5