Hotspot

trnS-GCU-trnG-UCC

LSC · 8411-9537 · 1127 bp

Back to Euterpe

Pi 0.0157

MarkerSeek score 69.50

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1127
variable_sites: 26
pi: 0.0157
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	45.0	38.1	58.0	55.5	1201-1506 bp	1.0	53.4
trnS-GCU-trnG-UCC_p2	ATTAGCAATCCGCCGCTTTA	AGAACGAATCACACTTTTACCAC	45.0	39.1	58.0	57.3	1204-1509 bp	1.0	52.4
trnS-GCU-trnG-UCC_p3	ATTAGCAATCCGCCGCTTTA	GAACGAATCACACTTTTACCACT	45.0	39.1	58.0	57.3	1203-1508 bp	1.0	52.4
trnS-GCU-trnG-UCC_p4	AGAAGAAGCGGAGAGAGAGG	ACGAATCACACTTTTACCACT	55.0	38.1	58.2	55.5	1261-1566 bp	1.0	51.7
trnS-GCU-trnG-UCC_p5	ATTAGCAATCCGCCGCTTTA	AACGAATCACACTTTTACCACT	45.0	36.4	58.0	56.2	1202-1507 bp	1.0	51.5