Hotspot

trnQ-UUG-psbK

LSC · 7130-7473 · 344 bp

Back to Euterpe

Pi 0.00389

MarkerSeek score 52.80

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 344
variable_sites: 2
pi: 0.00389
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	GAAGGATTCGAACCTCCGAG	GGGCAATTTGGCGAAGAAAA	55.0	45.0	57.8	57.8	487-489 bp	1.0	77.8
trnQ-UUG-psbK_p2	GATGGAAACGATCTGGGACG	GGGCAATTTGGCGAAGAAAA	55.0	45.0	58.4	57.8	507-509 bp	1.0	76.1
trnQ-UUG-psbK_p3	AGGATTCGAACCTCCGAGTA	GGGCAATTTGGCGAAGAAAA	50.0	45.0	57.6	57.8	485-487 bp	1.0	76.1
trnQ-UUG-psbK_p4	AAGGATTCGAACCTCCGAGT	GGGCAATTTGGCGAAGAAAA	50.0	45.0	58.4	57.8	486-488 bp	1.0	76.0
trnQ-UUG-psbK_p5	GAGTAACGGGACCAAAACCC	GGGCAATTTGGCGAAGAAAA	55.0	45.0	58.5	57.8	470-472 bp	1.0	75.8