Hotspot

ycf1

SSC · 126785-132412 · 5628 bp

Back to Eurya

Pi 0.000426
MarkerSeek score 45.30
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5628
variable_sites
5
pi
0.000426
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TTCTGGGAATATTAATGCTAGA AGTGACTCCAGGAATAAATGT 31.8 38.1 52.1 54.2 194 bp 1.0 40.8
ycf1_p2 TTCTGGGAATATTAATGCTAGA ACTCCAGGAATAAATGTTAGTCCT 31.8 37.5 52.1 57.1 190 bp 1.0 40.8
ycf1_p3 TTCTGGGAATATTAATGCTAGA AAGTGACTCCAGGAATAAATGT 31.8 36.4 52.1 55.1 195 bp 1.0 40.8
ycf1_p4 TTCTGGGAATATTAATGCTAGA AGTGACTCCAGGAATAAATGTT 31.8 36.4 52.1 55.1 194 bp 1.0 40.8
ycf1_p5 TTCTGGGAATATTAATGCTAGAT AGTGACTCCAGGAATAAATGT 30.4 38.1 52.8 54.2 194 bp 1.0 40.8