Hotspot

rpoC2

LSC · 17684-21856 · 4173 bp

Back to Eurya

Pi 0.000431
MarkerSeek score 46.60
Primer available yes
Label rpoC2

Feature Metadata

label_name
rpoC2
feature_type
gene
region
LSC
length_bp
4173
variable_sites
4
pi
0.000431
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC2_p1 ATGTTACTGCATGGATCGGG ATTAGGAATTCGTTGGGCCC 50.0 50.0 57.7 57.9 200 bp 1.0 78.2
rpoC2_p2 ATGTTACTGCATGGATCGGG GGCCCTTTAGGAACAGCTTT 50.0 50.0 57.7 58.1 185 bp 1.0 78.2
rpoC2_p3 ATGTTACTGCATGGATCGGG AATTCGTTGGGCCCTTTAGG 50.0 50.0 57.7 58.2 194 bp 1.0 77.5
rpoC2_p4 ATGTTACTGCATGGATCGGG GGAATTCGTTGGGCCCTTTA 50.0 50.0 57.7 58.2 196 bp 1.0 77.5
rpoC2_p5 GTTACTGCATGGATCGGGAT ATTAGGAATTCGTTGGGCCC 50.0 50.0 57.4 57.9 198 bp 1.0 75.9