Hotspot

rpoC1

LSC · 22022-24834 · 2813 bp

Back to Eurya

Pi 0.000355
MarkerSeek score 45.00
Primer available yes
Label rpoC1

Feature Metadata

label_name
rpoC1
feature_type
gene
region
LSC
length_bp
2813
variable_sites
2
pi
0.000355
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC1_p1 TTCCCACGGGTCTCATAGAA TTGCTCGTTCGAGAACTACG 50.0 50.0 57.8 58.0 2955 bp 1.0 77.8
rpoC1_p2 TAGTTGAACCGGAGAGACCC TTGCTCGTTCGAGAACTACG 55.0 50.0 58.4 58.0 2978 bp 1.0 76.2
rpoC1_p3 ATTCCCACGGGTCTCATAGA TTGCTCGTTCGAGAACTACG 50.0 50.0 57.5 58.0 2956 bp 1.0 76.0
rpoC1_p4 TCCCACGGGTCTCATAGAAT TTGCTCGTTCGAGAACTACG 50.0 50.0 57.5 58.0 2954 bp 1.0 76.0
rpoC1_p5 TTCCCACGGGTCTCATAGAA TTCGATTGCTCGTTCGAGAA 50.0 45.0 57.8 57.7 2960 bp 1.0 75.4