Hotspot

ycf3-trnS-GGA

LSC · 46047-46867 · 821 bp

Back to Eurya

Pi 0.00171
MarkerSeek score 49.30
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
821
variable_sites
3
pi
0.00171
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TTTCCATTTGTCCGCGATCT CCCTCGGTAAACAAAAGCCT 45.0 50.0 57.9 58.1 914 bp 1.0 78.6
ycf3-trnS-GGA_p2 TTTCCATTTGTCCGCGATCT ACGGAAAGAGAGGGATTCGA 45.0 50.0 57.9 58.2 935 bp 1.0 78.2
ycf3-trnS-GGA_p3 TTTCCATTTGTCCGCGATCT TTCCAATGCTACGCCTTGAA 45.0 45.0 57.9 57.8 885 bp 1.0 77.9
ycf3-trnS-GGA_p4 TTTCCATTTGTCCGCGATCT ACCCTCGGTAAACAAAAGCC 45.0 50.0 57.9 58.4 915 bp 1.0 76.3
ycf3-trnS-GGA_p5 CCATTTGTCCGCGATCTAGG CCCTCGGTAAACAAAAGCCT 55.0 50.0 58.5 58.1 911 bp 1.0 75.5