Hotspot

trnT-GGU-psbD

LSC · 33331-34429 · 1099 bp

Back to Eurya

Pi 0.000382
MarkerSeek score 56.00
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1099
variable_sites
1
pi
0.000382
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.95
markerseek_score
56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG GGACCAGCCTACAAAAACGA 55.0 50.0 58.1 58.1 1201-1259 bp 1.0 82.5
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA GGACCAGCCTACAAAAACGA 50.0 50.0 58.1 58.1 1204-1262 bp 1.0 82.3
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG AAAGAGCAGTAAACCGGACC 55.0 50.0 58.1 57.8 1216-1274 bp 1.0 81.9
trnT-GGU-psbD_p4 TCAGTGGTAGAGTAACGCCA AAAGAGCAGTAAACCGGACC 50.0 50.0 58.1 57.8 1219-1277 bp 1.0 81.7
trnT-GGU-psbD_p5 GTGGTAGAGTAACGCCATGG ACAAAAACGAAACGGTCCCT 55.0 45.0 58.1 58.2 1191-1249 bp 1.0 81.4