Hotspot

trnC-GCA

LSC · 27940-28010 · 71 bp

Back to Euphrasia

Pi 0.0563

MarkerSeek score 64.40

Primer available yes

Label trnC-GCA

Feature Metadata

label_name: trnC-GCA
feature_type: tRNA
region: LSC
length_bp: 71
variable_sites: 6
pi: 0.0563
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA_p1	GCGCAAATCCACTCAAAACA	AAAGAGGGGGCTTGACTTTG	45.0	50.0	57.9	58.0	637 bp	0.666667	65.7
trnC-GCA_p2	GCGCAAATCCACTCAAAACA	CTTTGTGAAAGAGGGGGCTT	45.0	50.0	57.9	58.0	644 bp	0.666667	65.7
trnC-GCA_p3	GCGCAAATCCACTCAAAACA	AAGGGGATGGGGAACATGTA	45.0	50.0	57.9	58.0	805 bp	0.666667	65.7
trnC-GCA_p4	GCGCAAATCCACTCAAAACA	GGTGCAGAGTCAAAAAGGGA	45.0	50.0	57.9	58.0	921 bp	0.666667	65.5
trnC-GCA_p5	GCGCAAATCCACTCAAAACA	TCAACGGTGCAGAGTCAAAA	45.0	45.0	57.9	58.0	926 bp	0.666667	65.4