Hotspot

rpoC2

LSC · 16489-20625 · 4137 bp

Back to Euphrasia

Pi 0.0196
MarkerSeek score 53.40
Primer available yes
Label rpoC2

Feature Metadata

label_name
rpoC2
feature_type
gene
region
LSC
length_bp
4137
variable_sites
119
pi
0.0196
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC2_p1 TGTGAGATTTTCCATCGTTT GCTCTCTGCTAATGGATCGG 35.0 55.0 53.1 57.9 203 bp 1.0 52.3
rpoC2_p2 TTGTGAGATTTTCCATCGTT GCTCTCTGCTAATGGATCGG 35.0 55.0 53.1 57.9 204 bp 1.0 52.3
rpoC2_p3 TGTGAGATTTTCCATCGTTT AATGGATCGGATTGCACCAA 35.0 45.0 53.1 57.9 193 bp 1.0 52.0
rpoC2_p4 TTGTGAGATTTTCCATCGTT AATGGATCGGATTGCACCAA 35.0 45.0 53.1 57.9 194 bp 1.0 52.0
rpoC2_p5 TTGTGAGATTTTCCATCGTTT GCTCTCTGCTAATGGATCGG 33.3 55.0 54.0 57.9 204 bp 1.0 52.0