Hotspot

rpoC1

LSC · 20727-23594 · 2868 bp

Back to Euphrasia

Pi 0.0155

MarkerSeek score 56.90

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2868
variable_sites: 66
pi: 0.0155
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	CTGCTGAGTACGATTCGACA	AGGAAGGAAGCTTAATCGGA	50.0	45.0	57.5	55.9	2995 bp	1.0	64.9
rpoC1_p2	TTCTCGATCTTGATTCGCGT	AGGAAGGAAGCTTAATCGGA	45.0	45.0	57.4	55.9	2942 bp	1.0	64.8
rpoC1_p3	CTGCTGAGTACGATTCGACA	AGGAAGGAAGCTTAATCGGAA	50.0	42.9	57.5	56.7	2995 bp	1.0	63.1
rpoC1_p4	TTCTCGATCTTGATTCGCGT	AGGAAGGAAGCTTAATCGGAA	45.0	42.9	57.4	56.7	2942 bp	1.0	63.0
rpoC1_p5	TCTGCTGAGTACGATTCGAC	AGGAAGGAAGCTTAATCGGA	50.0	45.0	57.2	55.9	2996 bp	1.0	62.6