Hotspot

atpF-atpH

LSC · 13029-13380 · 352 bp

Back to Euphrasia

Pi 0.0426

MarkerSeek score 68.10

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 352
variable_sites: 22
pi: 0.0426
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GAAAGAATCGGTTACATTTTTCA	AGAGGGAAAAATACGGGGGA	30.4	50.0	53.9	58.0	488 bp	1.0	47.6
atpF-atpH_p2	GAAAGAATCGGTTACATTTTTCA	GAGGGAAAAATACGGGGGAC	30.4	55.0	53.9	58.0	487 bp	1.0	47.6
atpF-atpH_p3	GAAAGAATCGGTTACATTTTTCA	GGGAAAAATACGGGGGACTC	30.4	55.0	53.9	58.0	485 bp	1.0	47.6
atpF-atpH_p4	GAAAGAATCGGTTACATTTTTCA	CAGAGGGAAAAATACGGGGG	30.4	55.0	53.9	57.9	489 bp	1.0	47.6
atpF-atpH_p5	GAAAGAATCGGTTACATTTTTCA	AGGGAAAAATACGGGGGACT	30.4	50.0	53.9	58.3	486 bp	1.0	47.6