Hotspot

ycf1

IRb · 117128-118294 · 1167 bp

Back to Euphorbia

Pi 0.0392

MarkerSeek score 66.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 1167
variable_sites: 229
pi: 0.0392
unique_haplotype_count: 51
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GAGCTACTGAGGAACAACGG	AGCGGTCGCATTTCTTCTTA	55.0	45.0	58.0	57.6	1374-2638 bp	0.898551	84.9
ycf1_p2	GAGCTACTGAGGAACAACGG	CAATGGAGTCGGTGTTACGA	55.0	50.0	58.0	57.6	1666-1815 bp	0.086957	47.2
ycf1_p3	GAGCTACTGAGGAACAACGG	TCAATGGAGTCGGTGTTACG	55.0	50.0	58.0	57.6	1667-1816 bp	0.086957	47.2
ycf1_p4	CCCCTTTGGTGTCATTGACA	CAATGGAGTCGGTGTTACGA	50.0	50.0	58.0	57.6	1461-1610 bp	0.072464	42.6
ycf1_p5	CCCCTTTGGTGTCATTGACA	TCAATGGAGTCGGTGTTACG	50.0	50.0	58.0	57.6	1462-1611 bp	0.072464	42.6