Hotspot

rpl16

LSC · 88348-89953 · 1606 bp

Back to Euphorbia

Pi 0.04

MarkerSeek score 77.80

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1606
variable_sites: 403
pi: 0.04
unique_haplotype_count: 66
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ACACATCAATTCTCGGGCTC	AATTGCACGAGTCGAATGGA	50.0	45.0	58.0	57.9	2070-2383 bp	0.956522	89.3
rpl16_p2	ACACATCAATTCTCGGGCTC	TTGCACGAGTCGAATGGATT	50.0	45.0	58.0	57.9	2068-2381 bp	0.956522	89.3
rpl16_p3	CTGCTTCCTTGATCACAGCA	TTGCACGAGTCGAATGGATT	50.0	45.0	58.2	57.9	2144-2457 bp	0.985507	89.2
rpl16_p4	AGCAACAATAACGTCACCGA	AATTGCACGAGTCGAATGGA	45.0	45.0	57.8	57.9	2130-2443 bp	0.942029	87.7
rpl16_p5	AGCAACAATAACGTCACCGA	TTGCACGAGTCGAATGGATT	45.0	45.0	57.8	57.9	2128-2441 bp	0.942029	87.7