Hotspot

rbcL-accD

LSC · 60934-61724 · 791 bp

Back to Euphorbia

Pi 0.0476

MarkerSeek score 75.90

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 791
variable_sites: 207
pi: 0.0476
unique_haplotype_count: 55
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TTGAATGCTACTGCGGGTAC	AATGTCCCTGACACCGACTA	50.0	50.0	58.0	58.1	1598-1718 bp	0.942029	89.0
rbcL-accD_p2	TTGAATGCTACTGCGGGTAC	ATGTCCCTGACACCGACTAA	50.0	50.0	58.0	58.1	1597-1717 bp	0.942029	89.0
rbcL-accD_p3	AATGAGGGACGTGATCTTGC	ATGTCCCTGACACCGACTAA	50.0	50.0	58.0	58.1	1021-1141 bp	0.927536	88.3
rbcL-accD_p4	AATGAGGGACGTGATCTTGC	TCGCTTTCACCACAAACGTA	50.0	45.0	58.0	58.1	1429-1501 bp	0.086957	51.2
rbcL-accD_p5	TTGAATGCTACTGCGGGTAC	TCGCTTTCACCACAAACGTA	50.0	45.0	58.0	58.1	2005-2077 bp	0.086957	49.5