Hotspot

ndhC-trnV-UAC

LSC · 54420-55632 · 1213 bp

Back to Euphorbia

Pi 0.0636

MarkerSeek score 80.70

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1213
variable_sites: 461
pi: 0.0636
unique_haplotype_count: 67
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GCCCCCATCGCAATAACATA	TAGTGCTCTCGGATCGGAAT	50.0	50.0	58.1	58.0	2137-2752 bp	0.913043	87.6
ndhC-trnV-UAC_p2	ATTGTAACCAAGCATCGCCT	TAGTGCTCTCGGATCGGAAT	45.0	50.0	57.9	58.0	1569-2184 bp	0.913043	87.4
ndhC-trnV-UAC_p3	TGGTTCAGGCATTTGCTCAT	TAGTGCTCTCGGATCGGAAT	45.0	50.0	58.1	58.0	350-2697 bp	0.130435	56.5
ndhC-trnV-UAC_p4	TGGTTCAGGCATTTGCTCAT	TGTATCACAAACCCAAGGGC	45.0	50.0	58.1	58.1	796-2601 bp	0.086957	53.3
ndhC-trnV-UAC_p5	TGGTTCAGGCATTTGCTCAT	CGGGGCAATTTAGGTATCCC	45.0	55.0	58.1	58.1	2410-2429 bp	0.057971	42.0