Hotspot

rps4-trnT-UGU

LSC · 50340-50778 · 439 bp

Back to Euphorbia

Pi 0.0592

MarkerSeek score 77.40

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 439
variable_sites: 150
pi: 0.0592
unique_haplotype_count: 48
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	CCCCAGACGGCGTATTTTT	CTCAGAGGTTAGAGCATCGC	52.6	55.0	58.1	57.9	527-604 bp	1.0	81.8
rps4-trnT-UGU_p2	CCCCAGACGGCGTATTTTT	GATGGTCATCGGTTCGACTC	52.6	55.0	58.1	58.2	496-573 bp	1.0	81.6
rps4-trnT-UGU_p3	AGTTAGTCCCGGTAAAGCCC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	59.1	57.9	545-622 bp	0.985507	81.6
rps4-trnT-UGU_p4	AGTTAGTCCCGGTAAAGCCC	GATGGTCATCGGTTCGACTC	55.0	55.0	59.1	58.2	514-591 bp	0.985507	81.4
rps4-trnT-UGU_p5	AGTTAGTCCCGGTAAAGCCC	ATTTCTGGTATGTGAGCCCG	55.0	50.0	59.1	57.7	570-647 bp	0.971014	79.4