Hotspot

psaA-pafI

LSC · 45863-46943 · 1081 bp

Back to Euphorbia

Pi 0.063

MarkerSeek score 74.40

Primer available yes

Label psaA-pafI

Feature Metadata

label_name: psaA-pafI
feature_type: igs
region: LSC
length_bp: 1081
variable_sites: 387
pi: 0.063
unique_haplotype_count: 60
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-pafI_p1	AGAGTTGGCCAAAATGAGCA	GAATTGACTGCTGAGCCGTA	45.0	50.0	58.0	58.0	1215-1643 bp	1.0	91.9
psaA-pafI_p2	ATGCCACTCAGCCAAAGAAA	GAATTGACTGCTGAGCCGTA	45.0	50.0	58.0	58.0	1243-1671 bp	1.0	91.9
psaA-pafI_p3	AGAGTTGGCCAAAATGAGCA	CTGAGCCGTATGAGGTAGGA	45.0	55.0	58.0	58.0	1205-1633 bp	1.0	91.9
psaA-pafI_p4	ATGCCACTCAGCCAAAGAAA	CTGAGCCGTATGAGGTAGGA	45.0	55.0	58.0	58.0	1233-1661 bp	1.0	91.9
psaA-pafI_p5	ACATGTACTTGATGTCCCGC	GAATTGACTGCTGAGCCGTA	50.0	50.0	58.0	58.0	1630-2058 bp	1.0	91.7