Hotspot

trnT-GGU-psbD

LSC · 33929-35724 · 1796 bp

Back to Euphorbia

Pi 0.0464

MarkerSeek score 81.90

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1796
variable_sites: 541
pi: 0.0464
unique_haplotype_count: 64
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1702-2241 bp	0.985507	88.9
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1705-2244 bp	0.985507	88.8
trnT-GGU-psbD_p3	GAGCCCCTTTAACTCAGTGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	57.9	58.2	1718-2257 bp	0.985507	88.5
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	CAAAAACGAAACGGTCCCTC	55.0	50.0	58.1	57.6	1701-2240 bp	0.971014	87.2
trnT-GGU-psbD_p5	TCAGTGGTAGAGTAACGCCA	CAAAAACGAAACGGTCCCTC	50.0	50.0	58.1	57.6	1704-2243 bp	0.971014	87.0