Hotspot

trnG-UCC-trnR-UCU

LSC · 10745-11620 · 876 bp

Back to Euphorbia

Pi 0.111

MarkerSeek score 78.00

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 876
variable_sites: 443
pi: 0.111
unique_haplotype_count: 65
primer_available: yes
alignment_reliability: 0.74
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	TGGAGAAAAATTGCGTCCAA	50.0	40.0	58.0	56.2	666-1228 bp	0.985507	76.4
trnG-UCC-trnR-UCU_p2	CCTAGCCTTCCAAGCTAACG	TGGAGAAAAATTGCGTCCAA	55.0	40.0	58.1	56.2	669-1231 bp	0.985507	76.2
trnG-UCC-trnR-UCU_p3	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	619-1181 bp	1.0	75.3
trnG-UCC-trnR-UCU_p4	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.1	56.9	622-1184 bp	1.0	75.1
trnG-UCC-trnR-UCU_p5	AGCCTTCCAAGCTAACGATG	ATGGAGAAAAATTGCGTCCA	50.0	40.0	58.0	55.9	667-1229 bp	0.985507	74.6