Hotspot

ycf3

LSC · 41813-43773 · 1961 bp

Back to Eupatorium

Pi 0.00205

MarkerSeek score 59.50

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1961
variable_sites: 6
pi: 0.00205
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	ACTGATGGGACCAACAAACA	TCCCGCGAGAAGTAATGAGA	45.0	50.0	57.3	58.2	2104-2113 bp	1.0	72.7
ycf3_p2	ACTGATGGGACCAACAAACA	GGATCATTTTCCCGCGAGAA	45.0	50.0	57.3	58.3	2113-2122 bp	1.0	71.9
ycf3_p3	ACTGATGGGACCAACAAACA	ATCATTTTCCCGCGAGAAGT	45.0	45.0	57.3	57.6	2111-2120 bp	1.0	71.4
ycf3_p4	ACTGATGGGACCAACAAACAA	TCCCGCGAGAAGTAATGAGA	42.9	50.0	58.0	58.2	2104-2113 bp	1.0	70.4
ycf3_p5	TGACTGATGGGACCAACAAA	TCCCGCGAGAAGTAATGAGA	45.0	50.0	57.0	58.2	2106-2115 bp	1.0	70.3