Hotspot

rpoB

LSC · 12717-15899 · 3183 bp

Back to Eupatorium

Pi 0.00126

MarkerSeek score 56.10

Primer available yes

Label rpoB

Feature Metadata

label_name: rpoB
feature_type: gene
region: LSC
length_bp: 3183
variable_sites: 6
pi: 0.00126
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB_p1	TGATGCGAATCGGGCTTTAA	ACCTGGGGTTTTTGATGCAT	45.0	45.0	58.0	58.0	267 bp	1.0	80.1
rpoB_p2	TGCGAATCGGGCTTTAATGA	ACCTGGGGTTTTTGATGCAT	45.0	45.0	58.0	58.0	264 bp	1.0	80.1
rpoB_p3	TGCGAATCGGGCTTTAATGA	AACCTGGGGTTTTTGATGCA	45.0	45.0	58.0	58.2	265 bp	1.0	78.8
rpoB_p4	TGATGCGAATCGGGCTTTAA	AACCTGGGGTTTTTGATGCA	45.0	45.0	58.0	58.2	268 bp	1.0	78.7
rpoB_p5	ATGATGCGAATCGGGCTTTA	ACCTGGGGTTTTTGATGCAT	45.0	45.0	57.7	58.0	268 bp	1.0	78.3