Hotspot

ccsA

SSC · 112387-113355 · 969 bp

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Pi 0.00344

MarkerSeek score 60.90

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 5
pi: 0.00344
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TCAATTCCCCTTTTCTATTTCT	TGATTCACATGGTTCTCGCC	31.8	50.0	53.0	58.3	1138 bp	1.0	40.4
ccsA_p2	TCAATTCCCCTTTTCTATTTCTT	TGATTCACATGGTTCTCGCC	30.4	50.0	53.9	58.3	1138 bp	1.0	40.4
ccsA_p3	TTCAATTCCCCTTTTCTATTTCT	TGATTCACATGGTTCTCGCC	30.4	50.0	53.9	58.3	1139 bp	1.0	40.4
ccsA_p4	TCAATTCCCCTTTTCTATTTCT	GAGAACCGCGTGAATCACTA	31.8	50.0	53.0	57.7	1165 bp	1.0	40.3
ccsA_p5	TCAATTCCCCTTTTCTATTTCTT	GAGAACCGCGTGAATCACTA	30.4	50.0	53.9	57.7	1165 bp	1.0	40.3