Hotspot

trnR-ACG-rrn5

IRa · 127833-128096 · 264 bp

Back to Eupatorium

Pi 0.00515
MarkerSeek score 57.50
Primer available yes
Label trnR-ACG-rrn5

Feature Metadata

label_name
trnR-ACG-rrn5
feature_type
igs
region
IRa
length_bp
264
variable_sites
2
pi
0.00515
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-rrn5_p1 CCACGTGCTCTAATCCTCTG GGTGACGATACTGTAGGGGA 55.0 55.0 57.8 57.9 343-348 bp 1.0 79.4
trnR-ACG-rrn5_p2 CCACGTGCTCTAATCCTCTG AATCCATCCCGAACTTGGTG 55.0 50.0 57.8 57.9 378-383 bp 1.0 78.7
trnR-ACG-rrn5_p3 CCACGTGCTCTAATCCTCTG ACCAATCCATCCCGAACTTG 55.0 50.0 57.8 57.9 381-386 bp 1.0 78.7
trnR-ACG-rrn5_p4 CCACGTGCTCTAATCCTCTG CAATCCATCCCGAACTTGGT 55.0 50.0 57.8 57.9 379-384 bp 1.0 78.7
trnR-ACG-rrn5_p5 CCACGTGCTCTAATCCTCTG CACCAATCCATCCCGAACTT 55.0 50.0 57.8 57.9 382-387 bp 1.0 78.7