Hotspot

ndhF-rpl32

SSC · 110312-111284 · 973 bp

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Pi 0.00411

MarkerSeek score 61.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 973
variable_sites: 6
pi: 0.00411
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	CGCCCCATATCCTTTCCTTT	50.0	50.0	58.0	57.6	1152 bp	1.0	77.2
ndhF-rpl32_p2	TGCCGTCGGAAAAAGAAGAA	CGCCCCATATCCTTTCCTTT	45.0	50.0	58.0	57.6	1150 bp	1.0	77.1
ndhF-rpl32_p3	TTGCCGTCGGAAAAAGAAGA	CGCCCCATATCCTTTCCTTT	45.0	50.0	58.0	57.6	1151 bp	1.0	77.1
ndhF-rpl32_p4	GTTGCCGTCGGAAAAAGAAG	TGACAAAGCTTTTAACGCCG	50.0	45.0	58.0	57.6	1170 bp	1.0	77.0
ndhF-rpl32_p5	TGCCGTCGGAAAAAGAAGAA	TGACAAAGCTTTTAACGCCG	45.0	45.0	58.0	57.6	1168 bp	1.0	76.9